Back to Search Start Over

A Portable Device for Simple Exosome Separation from Biological Samples.

Authors :
Chen, Wenwen
Xie, Yingying
Chang, Yuang
Xu, Yuhai
Zhao, Mengqian
Deng, Pengwei
Qin, Jianhua
Li, Hongjing
Source :
Micromachines; Oct2021, Vol. 12 Issue 10, p1182, 1p
Publication Year :
2021

Abstract

Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2072666X
Volume :
12
Issue :
10
Database :
Complementary Index
Journal :
Micromachines
Publication Type :
Academic Journal
Accession number :
153309951
Full Text :
https://doi.org/10.3390/mi12101182