IL‐10 induces IgG4 production in NOD‐scid Il2rγnull mice humanized by engraftment of peripheral blood mononuclear cells.

(B-F) Frequencies at 21 days after PBMC engraftment in spleens of human huCD45+ (B), CD3+ T cells (C), CD19+ B lineage cells (D), CD19+CD138- B cells (E), and CD19+CD138+ plasma cells (F). IL-10 induces IgG4 production in NOD-scid Il2r ^null mice humanized by engraftment of peripheral blood mononuclear cells To differentiate between B cells and plasma cells, we gated B cells as CD19+CD138- (Figure 1E,G) and plasma cells as CD138+ (Figure 1E,H). [Extracted from the article]