MYCELIAL BIOMASS, EXO & ENDO POLYSACCHARIDES IN WILD STRAIN OF SPARASSIS CRISPA.

Submerged culture conditions were studied for the production of Exo & Endo polysaccharide (EPS, IPS) from Sparassis crispa. Maximum mycelial biomass was observed at 30 °C and pH 6.5. The best culture media for mycelial growth was MEB with 7.08± 0.15g/L biomass production at 10 days of incubation period. However 7 days was found to be sufficient for obtaining good amount of biomass as its rate of increase was high with a very low increase thereafter. Under optimized culture conditions, the maximum EPS concentration obtained was 3.25±0.55g/L at 100rpm while the IPS produced was 2.58±0.6g/L. [ABSTRACT FROM AUTHOR]