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Non‐invasive surveys of mammalian viruses using environmental DNA.

Authors :
Alfano, Niccolò
Dayaram, Anisha
Axtner, Jan
Tsangaras, Kyriakos
Kampmann, Marie‐Louise
Mohamed, Azlan
Wong, Seth T.
Gilbert, M. Thomas P.
Wilting, Andreas
Greenwood, Alex D.
Source :
Methods in Ecology & Evolution; Oct2021, Vol. 12 Issue 10, p1941-1952, 12p
Publication Year :
2021

Abstract

Environmental DNA (eDNA) and invertebrate‐derived DNA (iDNA) are used to survey biodiversity non‐invasively to mitigate difficulties in obtaining wildlife samples, particularly in remote areas or for rare species. Recently, eDNA/iDNA were used to monitor known wildlife pathogens; however, most wildlife pathogens are unknown and often evolutionarily divergent.To detect and identify known and novel mammalian viruses from eDNA/iDNA, we used a curated set of RNA oligonucleotides as viral baits in a hybridization capture system coupled with high‐throughput sequencing.We detected multiple known and novel mammalian RNA and DNA viruses from multiple viral families from both waterhole eDNA and leech‐derived iDNA. Congruence was found between detected hosts and viruses identified in leeches and waterholes.Our results demonstrate that eDNA/iDNA samples represent an effective non‐invasive resource for studying wildlife viral diversity and for detecting novel potentially zoonotic viruses prior to their emergence. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2041210X
Volume :
12
Issue :
10
Database :
Complementary Index
Journal :
Methods in Ecology & Evolution
Publication Type :
Academic Journal
Accession number :
152818917
Full Text :
https://doi.org/10.1111/2041-210X.13661