Microwave-assisted synthesis of catalpol perpropionylated product and its neuroprotective effect.

Using catalpol and propionyl anhydride as raw materials, pyridine as solvent and acid binding agent, catalpol perpropionyl product (CP-6) was synthesised by microwave-assisted method. The synthesis conditions were optimized by orthogonal test, and the neuroprotective activity was initially screened by the colorimetric method of the tetramethylazolyzol salt trace enzyme reaction. The test results showed that when the molar ratio of propionic anhydride to catalpol was 18:1, the reaction temperature in the microwave reactor was 80 °C, the reaction time was 6 h, and the mass concentration of catalpol in pyridine was 25 g/L, the relative content of CP-6 in the reaction system could reach 98. 51%. MTT method was used to screen the neuroprotective activity of CP-6, and the results showed that CP-6 had neuroprotective effect and may be a potential neuroprotective agent. [ABSTRACT FROM AUTHOR]