Evaluation of Ovarian Reserve by Using AMH Test before Collection the Oocytes for ICSI Technique.

This study was conducted on goats with the purpose to evaluate the ovarian reserve before females submit to the ova retrieval for intracytoplasmic sperm injection (ICSI). The study was conducted at the college of veterinary medicine -- the University of Baghdad and Middle East Labs - Baghdad (2019-2020). The study included thirty female goats (does) within the age of puberty and sexual maturity. The animals were divided into two groups: group I (< 4 years old), and group II (> 4 years old). A blood sample (6 ml) was collected from each animal through the jugular vein (before slaughter) in tubes containing anticoagulants and then sent to the laboratory for estimation of antimullerian hormone (AMH). The study showed significant increases in the level of AMH (4.02 ± 0.17) of the group I (< 4 years old) comparative with (2.22 ± 0.54) to the group II (> 4 years old) at the (<0.01), and this result relation with the number of oocytes obtained from ovarian samples of animals after slaughtering, it was found that the number and quality of oocytes increased in the group I (29 oocytes from a 15ovarian samples, compared with group II (16 oocytes from 15ovarian samples. This result explains the decrease in the ovarian reservoir of follicles with an increase in the age in the doe as a result of the decrease in the level of AMH in the serum, which is considered an indicator for knowing the ovarian reservoir from the follicles and determining the productive age of the ovaries. According to the results of this study, the decrease in the level of AMH in the serum is an indication of a decrease in the number of follicles on the surface of the ovaries, which negatively affects the fertility and productivity of the goats or may affect the fertilization rate and the number of fertilized embryos. The AMH test is considered one of the important tests that must be taken before oocyte collection to reduce the effort and costs involved in conducting any assisted reproduction technique that requires collecting the oocytes from the donor. [ABSTRACT FROM AUTHOR]