Effect of Allogenic Omental Graft on Esophegotomy Incision in Dogs Models.

The study aimed to evaluate the beneficial effect of allogenic omental graft on esophageal healing in dogs. Twenty healthy male adult dogs were used they were randomly divided into two equal groups (control group 10, treated group 10). Under aseptic technique 4 cm skin incision was made in the midline of the cervical(ventral) of animal by scalpel, after that the incision was sutured immediately by polyglycolic acid suture materials 0.3 USP used simple continous technique, and the omental graft extracted from same animals by laprotomy piece of greater omentum was then placed over the oesophagotomy incision of the experiment group and secured in place with tacks sutures, In the control group, the esophagotomy suture line was left without graft. Esophageal healing process follow up 1,2 and 3 weeks post operation by histopathological examination, the result display in treated group on the first week post operation development of collagen and lamina properia contain congested blood vessels with epithelial regeneration. 2 weeks post operation show the epithelium of the mucosa of the esophagus was formed by hyperplasia of squamous cells. The surface of these epithelia was covered by keratin with present of bundle of collagen and fibroblasts. 3 weeks post operation showed the epithelium of esophagus was covered by keratin, with the presence of blood capillaries and also, there was lymphocytic infiltration in the lamina properia with RBC. The tunica muscularis was consisting of skeletal muscle fibers, inner longitudinal, and outer circular, proliferation of fibroblast with deposition of collagen, while in control group histopathological examination in the first week show The epithelium of the esophagus was stratified squamous with the presence of keratin on its surface, 2 weeks post operation show The mucosa of the esophagus consisted of stratified squamous epithelium with a keratin layer on its surface. The lamina properia was composed of loose connective tissue with infiltration of many WBC and blood vessels, 3 weeks post operation show the basal layer of epithelium had many folds which are interdigitation with the underlying lamina properia, which appeared loose connective tissue with few numbers of fibroblasts and lymphocytes, the healing of treated group better than control group. [ABSTRACT FROM AUTHOR]