Fabrication and characterization of electrospun nanofibers of Hypophthalmichthys molitrix sarcoplasmic protein recovered by acid‐chitosan flocculation coupling treatment.

Fish sarcoplasmic proteins (FSP) account for 20%–40% of the muscle protein and its high‐value utilization has become an urgent problem to be solved. Here, directly freeze‐dried sarcoplasmic protein (DFSP) and chitosan flocculated sarcoplasmic protein (CFSP) nanofibers were successfully fabricated by electrospinning technology and characterized in terms of morphology, chemical interaction, surface and mechanical properties, and thermal stability. SDS‐PAGE indicated similar protein bands for both DFSP and CFSP. Scanning electron microscopy observation showed good spinnability for CFSP and DFSP. Fourier transform infrared spectrometry and X‐ray diffraction results unveiled an interaction between sarcoplasmic protein and chitosan in CFSP nannofiber. Furthermore, CFSP nanofiber exhibited better performance than DFSP nanofiber in surface hydrophobicity, thermal stability, elasticity and flexibility. These results suggest the potential application of FSP nanofiber in functional components or drugs delivery, contributing to high‐value utilization of FSP. [ABSTRACT FROM AUTHOR]