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Highly Sensitive Fluorescence Assay of Enterotoxin A in Milk Using Carbon Quantum Dots as a Fluorophore.
- Source :
- Food Analytical Methods; Sep2021, Vol. 14 Issue 9, p1815-1825, 11p
- Publication Year :
- 2021
-
Abstract
- Staphylococcal enterotoxin A (SEA) is an exotoxin that releases from gram-positive bacteria, and it is a source of food poisoning. Here, the luminescence method is introduced to quantify and detect SEA using carbon quantum dots (CQDs) as fluorophore; SEA antibody loaded Fe<subscript>3</subscript>O<subscript>4</subscript> nanoparticles (Fe<subscript>3</subscript>O<subscript>4</subscript>@Ab) are prepared for SEA extraction. SEA in standards and milk samples were conjugated with Fe<subscript>3</subscript>O<subscript>4</subscript>@Ab (Fe<subscript>3</subscript>O<subscript>4</subscript>@Ab@SEA), separated by a 1.4 T magnet, and dispersed in phosphate buffer saline. The fluorescence of CQDs is quenched when mixed with Fe<subscript>3</subscript>O<subscript>4</subscript>@Ab@SEA, without elution of SEAs from the Fe<subscript>3</subscript>O<subscript>4</subscript>@Ab@SEA. It is a fast and reliable method compared with the immunoassay counterpart. At optimum quantification conditions, a linear relationship between SEAs concentration and CQDs fluorescent quenching was observed from 4 to 2000 pg mL<superscript>−1</superscript> with the detection limit 2 pg mL<superscript>−1</superscript>. Repeatability and reproducibility were calculated as a percentage of relative standard deviations and were from 1.56 to 11.76%. Finally, the proposed method was applied to measure the SEA contents of four sterilized and homogenized milk samples without fat precipitations and have shown significant reliability. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19369751
- Volume :
- 14
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Food Analytical Methods
- Publication Type :
- Academic Journal
- Accession number :
- 152248672
- Full Text :
- https://doi.org/10.1007/s12161-021-02009-0