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Alteration of circulating cell‐free DNA level by external cephalic version: A potential biomarker for direct evaluation of placental damage.

Authors :
Yabuzaki, Keiko
Kamide, Taizan
Ejima, Ruriko
Tsuruoka, Yuto
Sato, Mariko
Kondo, Ibuki
Hasegawa, Akihiro
Sato, Taisuke
Samura, Osamu
Okamoto, Aikou
Source :
Journal of Obstetrics & Gynaecology Research; Sep2021, Vol. 47 Issue 9, p3144-3150, 7p
Publication Year :
2021

Abstract

Aim: To confirm that variations in cell‐free fetal DNA (cffDNA) are indicators of external placental damage, we quantitatively investigated cffDNA alterations in maternal peripheral blood during external cephalic version (ECV). Methods: We recruited 48 singleton pregnant women who underwent ECV in our hospital. Before and immediately after ECV, we harvested 10 ml of maternal peripheral blood samples for cffDNA analysis. cffDNA alterations were assessed based on the fetal fraction (FF) rate. We performed ECV without epidural anesthesia but administered epidural anesthesia if ECV was disrupted due to severe pain. Results: The FF increased from 22.9% ± 5.7% to 27.0% ± 5.7% (p < 0.05) after ECV. The FF increased in both successful (before, 24.4% ± 5.9%; after, 28.1% ± 5.9%; p < 0.05) and unsuccessful (before, 21.8% ± 3.8%; after, 27.3% ± 4.2%; p < 0.05) cases, as well as in patients who received epidural anesthesia (before, 23.9% ± 4.7%; after, 28.5% ± 4.4%; p < 0.05) or underwent ECV more than once (before, 23.5% ± 6.1%; after, 28.4% ± 5.3%; p < 0.05). Conclusions: FF alterations increased due to external stresses during ECV; the alterations were markedly greater when the strength and duration of external stress increased. These FF alterations may serve as potential biomarkers for the direct assessment of placental damage. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13418076
Volume :
47
Issue :
9
Database :
Complementary Index
Journal :
Journal of Obstetrics & Gynaecology Research
Publication Type :
Academic Journal
Accession number :
152230712
Full Text :
https://doi.org/10.1111/jog.14853