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Performance Comparison of Five SARS-CoV-2 Antibody Assays for Seroprevalence Studies.

Authors :
Younhee Park
Ki Ho Hong
Su-Kyung Lee
Jungwon Hyun
Eun-Jee Oh
Jaehyeon Lee
Hyukmin Lee
Sang Hoon Song
Seung-Jung Kee
Gye Cheol Kwon
Su Hwan Kim
Hyeon-Nam Do
Ah-Ra Kim
June-Woo Lee
Sung Soon Kim
Hyun Soo Kim
Source :
Annals of Laboratory Medicine; 2022, Vol. 42 Issue 1, p71-78, 12p
Publication Year :
2022

Abstract

Background: Seroprevalence studies of coronavirus disease 2019 (COVID-19) cases, including asymptomatic and past infections, are important to estimate the scale of the disease outbreak and to establish quarantine measures. We evaluated the clinical performance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody assays available in Korea for use in seroprevalence studies. Methods: The sensitivity, specificity, cross-reactivity, and interference of five SARS-CoV-2 antibody assays were evaluated using the following: 398 serum samples from confirmed COVID-19 patients, 510 negative control samples from before 2018 (pre-pandemic), 163 serum samples from patients with SARS, Middle East respiratory syndrome (MERS), and other viral infections, and five samples for the interference study. Results: The sensitivities of the five assays ranged from 92.2% to 98%, and their specificities, including cross-reactivity and interference, ranged from 97.5% to 100%. The agreement rates were excellent (kappa >0.9). Adjustment of the cutoff values could be considered through ROC curve analysis. The positive predictive values of the individual assays varied from 3.5% to 100% at a 0.1% prevalence but were as high as =95% when two assays were combined. Conclusions: The prevalence of COVID-19 in Korea is considered to be exceptionally low at present; thus, we recommend using a combination of two or more SARS-CoV-2 antibody assays rather than a single assay. These results could help select SARS-CoV-2 antibody assays for COVID-19 seroprevalence studies in Korea. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22343806
Volume :
42
Issue :
1
Database :
Complementary Index
Journal :
Annals of Laboratory Medicine
Publication Type :
Academic Journal
Accession number :
151908758
Full Text :
https://doi.org/10.3343/alm.2022.42.1.71