Retinol binding protein 1 affects Xenopus anterior neural development via all‐trans retinoic acid signaling.

Background: Retinol binding protein 1 (Rbp1) acts as an intracellular regulator of vitamin A metabolism and retinoid transport. In mice, Rbp1 deficiency decreases the capacity of hepatic stellate cells to take up all‐trans retinol and sustain retinyl ester stores. Furthermore, Rbp1 is crucial for visual capacity. Although the function of Rbp1 has been studied in the mature eye, its role during early anterior neural development has not yet been investigated in detail. Results: We showed that rbp1 is expressed in the eye, anterior neural crest cells (NCCs) and prosencephalon of the South African clawed frog Xenopus laevis. Rbp1 knockdown led to defects in eye formation, including microphthalmia and disorganized retinal lamination, and to disturbed induction and differentiation of the eye field, as shown by decreased rax and pax6 expression. Furthermore, it resulted in reduced rax expression in the prosencephalon and affected cranial cartilage. Rbp1 inhibition also interfered with neural crest induction and migration, as shown by twist and slug. Moreover, it led to a significant reduction of the all‐trans retinoic acid target gene pitx2 in NCC‐derived periocular mesenchyme. The Rbp1 knockdown phenotypes were rescued by pitx2 RNA co‐injection. Conclusion: Rbp1 is crucial for the development of the anterior neural tissue. Key Findings: Rbp1 is required for eye development.Rbp1 is required for neural crest development.Rbp1 is up‐stream of the all‐trans RA target gene pitx2. [ABSTRACT FROM AUTHOR]