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Modified self-obtained pooled sampling to screen for and infections in men who have sex with men.
- Source :
- Sexually Transmitted Infections; Aug2021, Vol. 97 Issue 5, p324-328, 5p
- Publication Year :
- 2021
-
Abstract
- <bold>Objectives: </bold>To assess whether pooled sample testing with nucleic acid amplification tests was a potential alternative to three single-site sample testing to screen for Chlamydia trachomatis and Neisseria gonorrhoeae infections in asymptomatic men who have sex with men.<bold>Methods: </bold>We prospectively compared pooled sample testing with single-site sample testing in asymptomatic MSM. Self-obtained paired rectal samples, one gargle sample and one first-void urine sample were collected from participants to generate two sets of samples: one for pooled sample testing and the other for single-site testing. We used modified pooled sampling, which is defined as the use of gargle samples, instead of swabs, for the pooled sample to test for pharyngeal infection.<bold>Results: </bold>This study included 513 MSM. The positive rates of C. trachomatis and N. gonorrhoeae were 20.3% and 11.7%, respectively, for single-site sample testing. Compared with the sensitivity of single-site testing as the gold standard, the sensitivities of pooled sample testing for C. trachomatis and N. gonorrhoeae were 94.2% (95% CI 88.0% to 97.3%) and 98.3% (95% CI 90.9% to 99.9%), respectively. The concordance rate and kappa coefficient were 98.3% (95% CI 96.7% to 99.2%) and 0.945 (95% CI 0.859 to 1.000), respectively, for C. trachomatis and 98.8% (95% CI 90.1% to 100%) and 0.943 (95% CI 0.857 to 1.000), respectively, for N. gonorrhoeae.<bold>Conclusions: </bold>The modified pooled sampling had a comparably high consistency with single-site sample testing. The results strongly suggest that the gargle sample is suitable as a part of pooled sample for STI screening of C. trachomatis and N. gonorrhoeae. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13684973
- Volume :
- 97
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Sexually Transmitted Infections
- Publication Type :
- Academic Journal
- Accession number :
- 151533394
- Full Text :
- https://doi.org/10.1136/sextrans-2020-054666