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A rapid near-patient detection system for SARS-CoV-2 using saliva.

A rapid near-patient detection system for SARS-CoV-2 using saliva.

Authors :
Toppings, Noah B.
Mohon, Abu Naser
Lee, Yoonjung
Kumar, Hitendra
Lee, Daniel
Kapoor, Ratik
Singh, Gurmukh
Oberding, Lisa
Abdullah, Omar
Kim, Keekyoung
Berenger, Byron M.
Pillai, Dylan R.
Source :
Scientific Reports; 6/28/2021, Vol. 11 Issue 1, p1-9, 9p
Publication Year :
2021

Abstract

The highly infectious nature of SARS-CoV-2 necessitates the use of widespread testing to control the spread of the virus. Presently, the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. This increases the turnaround time for getting test results. This study sought to develop a rapid, near-patient saliva-based test for COVID-19 (Saliva-Dry LAMP) with similar accuracy to that of standard RT-PCR tests. A lyophilized dual-target reverse transcription-loop-mediated isothermal amplification (RT-LAMP) test with fluorometric detection by the naked eye was developed. The assay relies on dry reagents that are room temperature stable. A device containing a centrifuge, heat block, and blue LED light system was manufactured to reduce the cost of performing the assay. This test has a limit of detection of 1 copy/µL and achieved a positive percent agreement of 100% [95% CI 88.43% to 100.0%] and a negative percent agreement of 96.7% [95% CI 82.78–99.92%] relative to a reference standard test. Saliva-Dry LAMP can be completed in 105 min. Precision, cross-reactivity, and interfering substances analysis met international regulatory standards. The combination of ease of sample collection, dry reagents, visual detection, low capital equipment cost, and excellent analytical sensitivity make Saliva-Dry LAMP particularly useful for resource-limited settings. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20452322
Volume :
11
Issue :
1
Database :
Complementary Index
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
151125201
Full Text :
https://doi.org/10.1038/s41598-021-92677-z