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Comparative Transcriptome Identifies Gene Expression Networks Regulating Developmental Pollen Abortion in Ogura Cytoplasmic Male Sterility in Chinese Cabbage (Brassica rapa ssp. pekinensis).

Authors :
Lijiao Hu
Xiaowei Zhang
Yuxiang Yuan
Zhiyong Wang
Shuangjuan Yang
Ruina Li
Nath, Ujjal Kumar
Yanyan Zhao
Baoming Tian
Gongyao Shi
Zhengqing Xie
Fang Wei
Xiaochun Wei
Source :
Horticulturae; Jun2021, Vol. 7 Issue 6, p1-14, 14p
Publication Year :
2021

Abstract

Ogura cytoplasmicmale sterility (Ogura CMS), originally identified in wild radish (Raphanus sativus), has enabled complete pollen sterility in Brassica plants, but the underlying mechanism in Ogura CMS Chinese cabbage (Brassica rapa ssp. pekinensis) remains unclear. In this study cytological analysis showed that during microsporogenesis the meiosis occurred normally, and the uninucleated pollens subsequently formed, but the development of both binucleated and trinucleated pollens was obviously disrupted due to defects of pollen mitosis in the Ogura CMS line (Tyms) compared with the corresponding maintainer line (231–330). In transcriptome profiling a total of 8052 differentially expressed genes (DEGs) were identified, among which 3890 were up-regulated and 4162 were downregulated at the pollen abortion stages in an Ogura CMS line. KOG cluster analysis demonstrated that a large number of DEGs were related to the cytoskeleton’s dynamics, which may account for the failure of pollen mitosis during development in the Ogura CMS line. The pivotal genes related to the phenylpropane synthesis pathway (PAL, 4CL and CAD) were significantly down-regulated, which probably affected the formation and disposition of anther lignin and sporopollenin, and eventually led to abnormality in the pollen exine structure. In addition, several key up-regulated genes (GPX7, G6PD and PGD1) related to the glutathione oxidation-reduction (REDOX) reaction indicated that the accumulation of peroxides in Ogura CMS lines during this period affected the pollen development. Taken together, this cytological and molecular evidence is expected to advance our understanding of pollen abortion induced by Ogura cytoplasmic action in Chinese cabbage. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23117524
Volume :
7
Issue :
6
Database :
Complementary Index
Journal :
Horticulturae
Publication Type :
Academic Journal
Accession number :
151114027
Full Text :
https://doi.org/10.3390/horticulturae7060157