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Pt(II)-Thiocarbohydrazone Complex as Cytotoxic Agent and Apoptosis Inducer in Caov-3 and HT-29 Cells through the P53 and Caspase-8 Pathways.
- Source :
- Pharmaceuticals (14248247); Jun2021, Vol. 14 Issue 6, p509, 1p
- Publication Year :
- 2021
-
Abstract
- In this study, a platinum(II) complex ([Pt(H<subscript>2</subscript>L)(PPh<subscript>3</subscript>)] complex) containing a thiocarbohydrazone as the ligand was tested as an anti-proliferative agent against ovarian adenocarcinoma (Caov-3) and human colorectal adenocarcinoma (HT-29) through MTT assays. Apoptotic markers were tested by the AO/PI double staining assay and DNA fragmentation test. Flow cytometry was conducted to measure cell cycle distribution, while the p53 and caspase-8 pathways were tested via immunofluorescence assay. Results demonstrated that the cytotoxic effect of the Pt(II)-thiocarbohydrazone complexes against Caov-3 and HT-29 cells was highly significant, and this effect triggered the activation of the p53 and caspase-8 pathways. Besides, apoptosis stimulated by the Pt(II)-thiocarbohydrazone complex was associated with cell cycle arrest at the G0/G1 phase. These findings suggest that the target complex inhibited the proliferation of Caov-3 and HT-29 cells, resulting in the arrest of the cell cycle and induction of apoptosis via the stimulation of the p53 and caspase-8 pathways. The present data suggests that the Pt(II)-thiocarbohydrazone complex could also be a promising chemotherapeutic agent for other types of cancer cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- CASPASES
CELL cycle
CANCER cells
APOPTOSIS
FLOW cytometry
CELL death
P53 antioncogene
Subjects
Details
- Language :
- English
- ISSN :
- 14248247
- Volume :
- 14
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Pharmaceuticals (14248247)
- Publication Type :
- Academic Journal
- Accession number :
- 151060031
- Full Text :
- https://doi.org/10.3390/ph14060509