Back to Search Start Over

A Transcriptomic Analysis of Gene Expression in Chieh-Qua in Response to Fusaric Acid Stress.

Authors :
Min Wang
Wenrui Liu
Jinqiang Yan
Piaoyun Sun
Feng Chen
Biao Jiang
Dasen Xie
Yu’e Lin
Qingwu Peng
Xiaoming He
Source :
Horticulturae; Apr2021, Vol. 7 Issue 4, p1-14, 14p
Publication Year :
2021

Abstract

Fusarium wilt results in undesirable effects on the quality and production of chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How). Fusaric acid (FA), a secondary metabolite of biotin produced by pathogens of genus Fusarium, induced resistant responses in chieh-qua; however, the physiological and molecular mechanism(s) of FA resistance remains largely unknown. In our study, ‘A39’ (FA-resistant cultivar) exhibited decreased malondialdehyde (MDA) content and increased superoxide dismutase (SOD) enzyme activity when exposed to FA compared with ‘H5’ (FA-susceptible cultivar). More apoptosis cells existed in ‘H5’ than ‘A39’ after 2 days of FA treatment. RNA-seq results revealed that a total of 2968 and 3931 differentially expressed genes (DEGs) were detected under normal conditions (1562 up-regulated and 1406 down-regulated) and FA treatment (2243 up-regulated and 1688 down-regulated), respectively. Interestingly, DEGs associated with pathogen-related protein and ethylene (ET) biosynthesis and signal pathways were most significantly changed during FA stress. Notably, several crucial genes encoding pathogenesis-related protein (CL4451.Contig2, CL2175.Contig4), peroxidase (Unigene49615 and CL11695.Contig2), and ET-responsive transcription factors (TFs) (CL9320.Contig1, CL9849.Contig3, CL6826.Contig2, CL919. Contig6, and CL518.Contig7) were specifically induced after FA treatment. Collectively, the study provides molecular data for isolating candidate genes involved in FA resistance, especially ET related genes in chieh-qua. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23117524
Volume :
7
Issue :
4
Database :
Complementary Index
Journal :
Horticulturae
Publication Type :
Academic Journal
Accession number :
150940934
Full Text :
https://doi.org/10.3390/horticulturae7040088