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A Novel Structure Harboring bla CTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China.
- Source :
- Antibiotics (2079-6382); Apr2021, Vol. 10 Issue 4, p387, 1p
- Publication Year :
- 2021
-
Abstract
- The aim of this study was to elucidate the prevalence of bla<subscript>CTX-M-27</subscript>-producing Escherichia coli and transmission mechanisms of bla<subscript>CTX-M-27</subscript> from swine farms in China. A total of 333 E. coli isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive E. coli were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried bla<subscript>CTX-M-27</subscript>, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried bla<subscript>CTX-M-27</subscript> genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The bla<subscript>CTX-M-27</subscript> genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with bla<subscript>CTX-M-27</subscript>-plasmids indicated that the tra-trb region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing bla<subscript>CTX-M-27</subscript> in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the bla<subscript>CTX-M-27</subscript> gene in E. coli. This study indicated that the predominant vehicle for bla<subscript>CTX-M-27</subscript> transmission has diversified over time and that control strategies to limit bla<subscript>CTX-M-27</subscript> transmission in farm animals are necessary. [ABSTRACT FROM AUTHOR]
- Subjects :
- ESCHERICHIA coli
PLASMIDS
SWINE farms
SWINE
Subjects
Details
- Language :
- English
- ISSN :
- 20796382
- Volume :
- 10
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Antibiotics (2079-6382)
- Publication Type :
- Academic Journal
- Accession number :
- 150857834
- Full Text :
- https://doi.org/10.3390/antibiotics10040387