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Immunogenicity of Adjuvanted Psoralen-Inactivated SARS-CoV-2 Vaccines and SARS-CoV-2 Spike Protein DNA Vaccines in BALB/c Mice.
- Source :
- Pathogens; May2021, Vol. 10 Issue 5, p626, 1p
- Publication Year :
- 2021
-
Abstract
- The development of a safe and effective vaccine to protect against COVID-19 is a global priority due to the current high SARS-CoV-2 infection rate. Currently, there are over 160 SARS-CoV-2 vaccine candidates at the clinical or pre-clinical stages of development. Of these, there are only three whole-virus vaccine candidates produced using β-propiolactone or formalin inactivation. Here, we prepared a whole-virus SARS-CoV-2 vaccine (SARS-CoV-2 PsIV) using a novel psoralen inactivation method and evaluated its immunogenicity in mice using two different adjuvants, alum and Advax-2. We compared the immunogenicity of SARS-CoV-2 PsIV against SARS-CoV-2 DNA vaccines expressing either full-length or truncated spike proteins. We also compared the psoralen-inactivated vaccine against a DNA prime, psoralen-inactivated vaccine boost regimen. After two doses, the psoralen-inactivated vaccine, when administered with alum or Advax-2 adjuvants, generated a dose-dependent neutralizing antibody responses in mice. Overall, the pattern of cytokine ELISPOT responses to antigen-stimulation observed in this study indicates that SARS-CoV-2 PsIV with the alum adjuvant promotes a Th2-type response, while SARS-CoV-2 PsIV with the Advax-2 adjuvant promotes a Th1-type response. [ABSTRACT FROM AUTHOR]
- Subjects :
- DNA vaccines
SARS-CoV-2
VACCINE effectiveness
DNA primers
COVID-19 pandemic
VACCINES
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 10
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 150501144
- Full Text :
- https://doi.org/10.3390/pathogens10050626