Introduction of OsMADS18 gene into west Nusa Tenggara local rice (Oryza sativa) cv. beak ganggas using agrobacterium-mediated transformation.

West Nusa Tenggara (NTB) local rice cultivar Beak ganggas is rice with functional food because of anthocyanin content. A long harvest time of Beak ganggas makes the rice to be rarely planted by local farmers. The introduction of the early flowering gene OsMADS18 through Agrobacterium-mediated transformation can be done to solve the drawback of Beak ganggas. A. tumefaciens as a transformant agent had been inserted in Beak ganggas genome. The result shows that from 50 calluses infected sprout up 42 calluses in hygromycin selection, 35 calluses regenerate into shoots and 20 plantlets. The calculation of transformation efficiency is 84%, while regeneration efficiency is 83%. Confirmation of Beag ganggas OsMADS18 is used hptII primer and OL13/14 primer, which produces 500 and 742 bp DNA fragment constitutively. So that, OsMADS18 has been successfully inserted in Beak Ganggas genome using Agrobacterium-mediated transformation. [ABSTRACT FROM AUTHOR]