Evaluating the granzyme production from CD8 T cells and NK1.1 natural killer cell after treating by the combination of Momordica charantia and Averrhoa bilimbi extract in STZ induced mice.

Recently, diabetes mellitus (DM) still become the top health problem worldwide. Several reports showed the incidence and death number of this disease are increasing every year. Therefore, the strategy to overcome DM is crucially needed. Another possible way to minimize the adverse effect of DM is by understanding the immunological activities and properties, which lead us to have another option or insight to treat DM. Thus, in this present study, we aim to evaluate the production of granzyme by CD8 T cells and NK.1.1 natural killer (NK) cells after treated by the combination of Momordica charantia and Averrhoa bilimbi extract in streptozotocin (STZ) induced mice. The animal mice model was firstly induced by STZ, then followed by the Momordica charantia and Averrhoa bilimbi extract treatment. Finally, the expression number of granzyme from CD8 T cells and NK1.1 NK cells was evaluated using flow cytometry (FACS Calibur^TM). According to our observation, we found that the granzyme is not produced by both CD8 T cells and NK1.1 NK cells, even after treated by the Momordica charantia and Averrhoa bilimbi extract. [ABSTRACT FROM AUTHOR]