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Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef.
- Source :
- Acta Alimentaria; Jun2021, Vol. 50 Issue 2, p170-179, 10p
- Publication Year :
- 2021
-
Abstract
- A total of 46 Staphylococcus aureus isolates from fish and ground beef were tested for the agr types, icaABCD genes, and biofilm formation at 12, 25 and 37 °C by the microtiter plate and the MTT assays. All isolates were positive for the icaABD genes, while 97.8% were positive for the icaC. All isolates produced biofilms at 37 and 25 °C, but 93.5% of them were also biofilm producers at 12 °C. There was no significant difference in biofilm formation between 25 and 37 °C using the crystal violet assay (P > 0.05). However, statistically significant differences were detected between 12 and 25 °C as well as 12 and 37 °C (P < 0.05). All isolates were significantly different in biofilm production by the MTT assay at all tested temperatures. Furthermore, a relationship between the presence of the icaABCD genes and biofilm formation was observed. The agr type I was the most prevalent (54.4%) among the isolates, followed by agr type II (41.3%) and agr type III (9.6%). In this study, the S. aureus isolates exhibited biofilm formation ability responsible for persistence of bacteria in foods, which may lead to food spoilage and human health problems. [ABSTRACT FROM AUTHOR]
- Subjects :
- GROUNDFISHES
STAPHYLOCOCCUS aureus
BIOFILMS
GENTIAN violet
Subjects
Details
- Language :
- English
- ISSN :
- 01393006
- Volume :
- 50
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Acta Alimentaria
- Publication Type :
- Academic Journal
- Accession number :
- 150426150
- Full Text :
- https://doi.org/10.1556/066.2020.00203