Dynamic Transcriptome Sequencing of Bovine Alphaherpesvirus Type 1 and Host Cells Carried Out by a Multi-Technique Approach.

The BoHV-1/HU sample [500 ng Poly(A)+ RNA] and the ONT Direct RNA Sequencing Kit (SQK-RNA001) were used for this experiment. Technical Validation and Data Re-Use The quantity of the samples was determined using Invitrogen Qubit 4 Fluorometer (Thermo Fisher Scientific) with Qubit RNA BR Assay Kit, Qubit RNA HS Assay Kit, or dsDNA HS Assay Kit (Thermo Fisher Scientific). Keywords: long-read sequencing technology; nanopore sequencing technology; synthetic long-read sequencing; herpesviruses; bovine herpesvirus; transcriptomics EN long-read sequencing technology nanopore sequencing technology synthetic long-read sequencing herpesviruses bovine herpesvirus transcriptomics N.PAG N.PAG 8 05/17/21 20210407 NES 210407 Introduction Bovine alphaherpesvirus type 1 (BoHV-1) is an alphaherpesvirus causing a disease known as infectious bovine rhinotracheitis (Thiry et al., [37]), which causes serious economic losses in cattle industry (van Oirschot, [45]). [Extracted from the article]