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A smart mitochondria-targeting TP-NIR fluorescent probe for the selective and sensitive sensing of H2S in living cells and mice.
- Source :
- New Journal of Chemistry; 4/28/2021, Vol. 45 Issue 16, p7315-7320, 6p
- Publication Year :
- 2021
-
Abstract
- Hydrogen sulfide (H<subscript>2</subscript>S) is one of the important gaseous signalling molecules, which plays key roles in various critical biological processes. In this work, we report a novel two-photon near-infrared (TP-NIR) fluorescent probe (MNIR-H<subscript>2</subscript>S) for the sensing of mitochondrial H<subscript>2</subscript>S in living cells, tissues, zebrafish and nude mice. Using a xanthene dye as a TP-excitation (810 nm) NIR-emission (668 nm) fluorophore, a well-known H<subscript>2</subscript>S response moiety with a robust intramolecular charger transfer (ICT) effect can quickly respond to H<subscript>2</subscript>S and effectively quench the fluorescence intensity, and an oxonium cation structure serves as a mitochondria-targeting site. Notably, MNIR-H<subscript>2</subscript>S displays high sensitivity, high selectivity, low cytotoxicity, powerful mitochondrial localization ability, deep tissue penetration, and a very minimal imaging background. Biological imaging results show the biological application of MNIR-H<subscript>2</subscript>S as a robust new tool for use in H<subscript>2</subscript>S imaging in biological systems. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 11440546
- Volume :
- 45
- Issue :
- 16
- Database :
- Complementary Index
- Journal :
- New Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 150036466
- Full Text :
- https://doi.org/10.1039/d1nj00840d