Screening and detection of transgenic components in 706 conventional soybean lines.

In order to investigate and analyze whether transgenic soybean lines are mixed into conventional soybean breeding in China, 706 soybean lines were selected for PCR amplification of several regulatory elements (CaMV35S promoter, FMV35S promoter and NOS terminator), as well as the main herbicide tolerant transgenic soybean transformants (GTS40-3-2 and MON89788) approved for import in China. Meanwhile, a specific qualitative PCR detection method for soybean event (MON89788) was established and 5' and 3' flanking sequences were designed. The products of 445 bp and 340 bp were amplified by primers, which can be applied to the detection of MON89788 soybean. Finally, of all the samples selected, the proportion of transgenic components containing CaMV35S promoter, FMV35S promoter, NOS terminator and herbicide tolerant transgenic line MON89788 was 1%, 0.85%, 0.71% and 0.85% respectively, so as to evaluate the proportion of transgenic soybeans and provide consumers with the share of transgenic soybeans. To provide a strong basis for the agricultural genetically modified soybean regulatory authorities to carry out monitoring work to provide data support. [ABSTRACT FROM AUTHOR]