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P25 Gene Knockout Contributes to Human Epidermal Growth Factor Production in Transgenic Silkworms.
- Source :
- International Journal of Molecular Sciences; Mar2021, Vol. 22 Issue 5, p2709-2709, 1p
- Publication Year :
- 2021
-
Abstract
- Transgenic silkworm expression systems have been applied for producing various recombinant proteins. Knocking out or downregulating an endogenous silk protein is considered a viable strategy for improving the ability of transgenic expression systems to produce exogenous proteins. Here, we report the expression of human epidermal growth factor (hEGF) in a P25 gene knockout silkworm. The hEGF gene regulated by the P25 gene promoter was integrated into a silkworm's genome. Five transgenic positive silkworm lineages were generated with different insertion sites on silkworm chromosomes and the ability to synthesize and secrete proteins into cocoons. Then, a cross-strategy was used to produce transgenic silkworms with a P25 gene knockout background. The results of the protein analysis showed that the loss of an endogenous P25 protein can increase the hEGF production to about 2.2-fold more than normal silkworms. Compared to those of transgenic silkworms with wild type (non-knockout) background, the morphology and secondary structure of cocoon silks were barely changed in transgenic silkworms with a P25 gene knockout background, indicating their similar physical properties of cocoon silks. In conclusion, P25 gene knockout silkworms may become an efficient bioreactor for the production of exogenous proteins and a promising tool for producing various protein-containing silk biomaterials. [ABSTRACT FROM AUTHOR]
- Subjects :
- GENE knockout
SILKWORMS
FACTORS of production
RECOMBINANT proteins
PROTEIN analysis
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 22
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 149241044
- Full Text :
- https://doi.org/10.3390/ijms22052709