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Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves.

Authors :
El Soury, Marwa
García-García, Óscar Darío
Moretti, Matteo
Perroteau, Isabelle
Raimondo, Stefania
Lovati, Arianna Barbara
Carriel, Víctor
Tonazzini, Ilaria
Ronchi, Giulia
Source :
International Journal of Molecular Sciences; Mar2021, Vol. 22 Issue 5, p2389-2389, 1p
Publication Year :
2021

Abstract

In critical nerve gap repair, decellularized nerve allografts are considered a promising tissue engineering strategy that can provide superior regeneration results compared to nerve conduits. Decellularized nerves offer a well-conserved extracellular matrix component that has proven to play an important role in supporting axonal guiding and peripheral nerve regeneration. Up to now, the known decellularized techniques are time and effort consuming. The present study, performed on rat sciatic nerves, aims at investigating a novel nerve decellularization protocol able to combine an effective decellularization in short time with a good preservation of the extracellular matrix component. To do this, a decellularization protocol proven to be efficient for tendons (DN-P1) was compared with a decellularization protocol specifically developed for nerves (DN-P2). The outcomes of both the decellularization protocols were assessed by a series of in vitro evaluations, including qualitative and quantitative histological and immunohistochemical analyses, DNA quantification, SEM and TEM ultrastructural analyses, mechanical testing, and viability assay. The overall results showed that DN-P1 could provide promising results if tested in vivo, as the in vitro characterization demonstrated that DN-P1 conserved a better ultrastructure and ECM components compared to DN-P2. Most importantly, DN-P1 was shown to be highly biocompatible, supporting a greater number of viable metabolically active cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16616596
Volume :
22
Issue :
5
Database :
Complementary Index
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
149240725
Full Text :
https://doi.org/10.3390/ijms22052389