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Effects of mineral complex material treatment on 2,4- dinitrochlorobenzene-induced atopic dermatitis like-skin lesions in mice model.
- Source :
- BMC Complementary Medicine & Therapies; 3/3/2021, Vol. 21 Issue 1, p1-13, 13p, 2 Color Photographs, 1 Diagram, 2 Charts, 4 Graphs
- Publication Year :
- 2021
-
Abstract
- Background: Atopic dermatitis (AD) is a chronic allergic inflammatory skin disease characterized by complex pathogenesis including skin barrier dysfunction, immune-redox disturbances, and pruritus. Prolonged topical treatment with medications such as corticosteroids, calcineurin inhibitors, and T-cell inhibitors may have some potential side-effects. To this end, many researchers have explored numerous alternative therapies using natural products and mineral compounds with antioxidant or immunomodulatory effects to minimize toxicity and adverse-effects. In the current study, we investigated the effects of mineral complex material (MCM) treatment on 2, 4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in SKH-1 hairless mice. Methods: Animals were divided into four groups; normal control (NC), negative control treated with DNCB only (DNCB only), positive control treated with DNCB and tacrolimus ointment (PC) and experimental group treated with DNCB and MCM patch (MCM). Skin inflammation and lesion severity were investigated through analyses of skin parameters (barrier score and strength, moisture and trans-epidermal water loss level), histopathology, immunoglobulin E, and cytokines. In addition, reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxidase (GPx), and catalase (CAT) levels were measured in both serum and skin lysate. Results: Our results demonstrates that MCM patch improved the progression of AD-like skin lesions by significantly increasing skin barrier strength and decreasing trans-epidermal water loss. Additionally, dermal administration of MCM patch significantly reduced epidermal thickness, ROS, and NO levels in skin lysate. Furthermore, we found that MCM suppressed the levels of AD-involved (Th<subscript>1</subscript> and Th<subscript>2</subscript>) cytokines such as IL-2, IFN-γ, and IL-4 in blood. In addition, the levels of other Th<subscript>1,</subscript> and Th<subscript>2</subscript> and inflammatory cytokines such as IL-1β, TNF-α, IL-6, IL-12(p70) and IL-10 were found lowest in the MCM group than in the DNCB only and PC groups. Moreover, we found total serum IgE level significantly increased after DNCB treatment, but decreased in the PC and MCM groups. Conclusion: Taken together, our findings suggest that MCM application may have beneficial effects either systemic or regional on DNCB-induced AD lesional skin via regulation of the skin barrier function and immune-redox response. [ABSTRACT FROM AUTHOR]
- Subjects :
- SKIN disease treatment
ATOPIC dermatitis treatment
THERAPEUTIC use of minerals
IMMUNOGLOBULIN analysis
ATOPIC dermatitis
BENZENE derivatives
BIOLOGICAL models
NITRIC oxide
DATA analysis
BODY weight
STATISTICAL sampling
SEVERITY of illness index
CATALASE
DESCRIPTIVE statistics
MICE
REACTIVE oxygen species
ANIMAL experimentation
TACROLIMUS
WATER-electrolyte balance (Physiology)
ANALYSIS of variance
STATISTICS
INFLAMMATION
CYTOKINES
GLUTATHIONE peroxidase
DATA analysis software
INTERLEUKINS
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 21
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 149129946
- Full Text :
- https://doi.org/10.1186/s12906-021-03259-5