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In vivo NIR-II structured-illumination light-sheet microscopy.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; 2/9/2021, Vol. 118 Issue 6, p1-10, 10p
- Publication Year :
- 2021
-
Abstract
- Noninvasive optical imaging with deep tissue penetration depth and high spatiotemporal resolution is important to longitudinally studying the biology at the single-cell level in live mammals, but has been challenging due to light scattering. Here, we developed near-infrared II (NIR-II) (1,000 to 1,700 nm) structured-illumination light-sheet microscopy (NIR-II SIM) with ultralong excitation and emission wavelengths up to ~1,540 and ~1,700 nm, respectively, suppressing light scattering to afford large volumetric threedimensional (3D) imaging of tissues with deep-axial penetration depths. Integrating structured illumination into NIR-II light-sheet microscopy further diminished background and improved spatial resolution by approximately twofold. In vivo oblique NIR-II SIM was performed noninvasively for 3D volumetric multiplexed molecular imaging of the CT26 tumor microenvironment in mice, longitudinally mapping out CD4, CD8, and OX40 at the single-cell level in response to immunotherapy by cytosine-phosphate-guanine (CpG), a Toll-like receptor 9 (TLR-9) agonist combined with OX40 antibody treatment. NIR-II SIM affords an additional tool for noninvasive volumetric molecular imaging of immune cells in live mammals. [ABSTRACT FROM AUTHOR]
- Subjects :
- MICROSCOPY
LIGHT scattering
TOLL-like receptors
OPTICAL images
CELL imaging
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 118
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 148712879
- Full Text :
- https://doi.org/10.1073/pnas.2023888118