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miR-29a is a negative regulator of influenza virus infection through targeting of the frizzled 5 receptor.
- Source :
- Archives of Virology; 2021, Vol. 166 Issue 2, p363-373, 11p
- Publication Year :
- 2021
-
Abstract
- Influenza A virus (IAV) infections result in a large number of deaths and substantial economic losses each year. MicroRNAs repress gene expression and are involved in virus-host interactions. miR-29a is known to have anti-tumor and anti-fibrotic effects. However, the role of miR-29a in IAV infection is unclear. In the present study, we investigated the effect of miR-29a on IAV infection and the mechanisms by which it functions. IAV infection was found to cause decreased miR-29a expression in lung epithelial A549 cells and mouse lungs. Overexpression of miR-29a reduced IAV mRNA and protein levels and progeny virus production in HEK293 and A549 cells. Inhibition of IAV infection by miR-29a was observed with different strains of IAV, including A/PR/8/34, A/WSN/1933, and clinical isolates A/OK/3052/09 and A/OK/309/06 H3N2. Knockout of miR-29a using CRISPR/Cas9 resulted in an increase in viral mRNA and protein levels, confirming that miR-29a suppresses IAV infection. A 3' untranslated region (3'-UTR) reporter assay showed that miR-29a had binding sites in the 3'-UTR of the Wnt-Ca<superscript>2+</superscript> signaling receptor frizzled 5 gene, and overexpression of miR-29a reduced the level of the endogenous frizzled 5 protein. Wnt5a treatment of HEK293 and A549 cells enhanced IAV infection. Our results suggest that miR-29a inhibits IAV infection, probably via the frizzled 5 receptor. [ABSTRACT FROM AUTHOR]
- Subjects :
- VIRUS diseases
INFLUENZA A virus
VIRAL proteins
BINDING sites
EPITHELIAL cells
Subjects
Details
- Language :
- English
- ISSN :
- 03048608
- Volume :
- 166
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Archives of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 148427042
- Full Text :
- https://doi.org/10.1007/s00705-020-04877-z