Detection of Antiseptic Resistant Genes in Colistin- Resistant Pseudomonas aeruginosa and MDR Klebsiella penumoniae.

Objectives: The aim of this study was to detect the existence of developments in resistance to biocide of Pseudomonas aeruginosa resist of colistin and in multi-drug resistance Klebsiella pneumonia in the hospital environment. Materials and Methods: The study included 25 isolates of K. pneumoniae and 30 isolates of P. aeruginosa. Isolated from different clinical and environmental samples in Baghdad hospitals. Antibiotic sensitivity tests and their susceptibility to multiple antibiotic resistance and sensitivity tests were studied for the most commonly used antiseptics at the preventive level (benzalkonium chloride). The test was carried out using the micro dilution broth method, following Institution of Clinical and Laboratory Standards guidelines, PCR was performed for detection of bla_TEM, bla_SHV, bla_CTX-M, qacC/D, qacΔE1and qacE beta lactamase and antiseptic genes. Results: A high rate of multiple resistance to the most used antibiotics was observed, so the rate of resistance to all antibiotics that was used was 16.0% of P. aeruginosa and 4% of K. pneumoniae possesses comprehensive resistance to all antibiotics that were used and the resiste of colistin in P. aeruginosa was 36% .The prevalence of ESBLs was 36.0% and 48.0 % of P.aeruginosa and K.penomoniea respectively,in addition to their strong ability to form biofilms 80 % in P.aeruginosa and 94% in k.pneumoniea and their ability to resist Antiseptics . The percentage of resistance to antiseptic of benzilkonium chloride showed the highest concentration of P.aeruginosa was 33.3% and K. pneumoniae 37.5% . The result of ESBL and antiseptic genes detection clarify, the percent of production genes were (10%), (40%) blaTEM; (6.66%),(56%)bla_SHV ;(33%),(64%) bla_CTX-M ;(70%),(44%) qacC/D; ( 80% ),(56%) qacΔE1 in P.aeruginosa and K.pneumoniea respectively and no any isolate carried qacE gene. production of extended spectrum β-lactamase genes in addition to their strong ability to form biofilms 80 % in p.aeroginosa and 94% in k.pneumoniea and their ability to resist a antiseptics . The percentage of resistance to antiseptic of benzilkonium chloride showed the highest concentration of P. aeruginosa was 33.3% and K. pneumoniae 37.5%, the result of beta lactamase and antiseptic genes detection clarify PCR was performed for detection of bla_TEM, bla_SHV, bla_CTX-M, qacC/D, qacΔE1and qacE beta lactamase and antiseptic genes . Conclusion: Our observations indicate that there is a significant correlation between the ability of bacteria to resist multiple antibiotics in addition to their ability to resist the most commonly used antiseptics, due to their physiological nature and increased virulence factors. [ABSTRACT FROM AUTHOR]