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Isolation, Identification, and Bioinformatic Analysis of Antibacterial Proteins and Peptides from Immunized Hemolymph of Red Palm Weevil Rhynchophorus ferrugineus.

Authors :
Knutelski, Stanisław
Awad, Mona
Łukasz, Natalia
Bukowski, Michał
Śmiałek, Justyna
Suder, Piotr
Dubin, Grzegorz
Mak, Paweł
Source :
Biomolecules (2218-273X); Jan2021, Vol. 11 Issue 1, p83, 1p
Publication Year :
2021

Abstract

Red palm weevil (Rhynchophorus ferrugineus Olivier, 1791, Coleoptera: Curculionidae) is a destructive pest of palms, rapidly extending its native geographical range and causing large economic losses worldwide. The present work describes isolation, identification, and bioinformatic analysis of antibacterial proteins and peptides from the immunized hemolymph of this beetle. In total, 17 different bactericidal or bacteriostatic compounds were isolated via a series of high-pressure liquid chromatography steps, and their partial amino acid sequences were determined by N-terminal sequencing or by mass spectrometry. The bioinformatic analysis of the results facilitated identification and description of corresponding nucleotide coding sequences for each peptide and protein, based on the recently published R. ferrugineus transcriptome database. The identified compounds are represented by several well-known bactericidal factors: two peptides similar to defensins, one cecropin-A1-like peptide, and one attacin-B-like protein. Interestingly, we have also identified some unexpected compounds comprising five isoforms of pheromone-binding proteins as well as seven isoforms of odorant-binding proteins. The particular role of these factors in insect response to bacterial infection needs further investigation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2218273X
Volume :
11
Issue :
1
Database :
Complementary Index
Journal :
Biomolecules (2218-273X)
Publication Type :
Academic Journal
Accession number :
148286308
Full Text :
https://doi.org/10.3390/biom11010083