Back to Search
Start Over
Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling.
- Source :
- Analytical & Bioanalytical Chemistry; 2021, Vol. 413 Issue 4, p1027-1037, 11p
- Publication Year :
- 2021
-
Abstract
- The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 (HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3-DNA target by the technique of square wave anodic stripping voltammetry (SWASV). The functionalized-PbS quantum dots were characterized by UV-vis, FTIR, XRD, TEM and zeta potential techniques. As-prepared, functionalized-PbS quantum dots have an average size of 4.15 ± 1.35 nm. The detection platform exhibited LOD and LOQ values of 1.23 fM and 2.11 fM, respectively. HEV3-DNA target spiked serum is also reported. Graphical abstract [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 413
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 148164118
- Full Text :
- https://doi.org/10.1007/s00216-020-03061-1