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Stimulatory effects of nano-selenium and conjugated linoleic acid on antioxidant activity, trace minerals, and gene expression response of growing male Moghani lambs.

Authors :
Ghaderzadeh, Shirzad
Aghjehgheshlagh, Farzad Mirzaei
Nikbin, Saeid
Navidshad, Bahman
Source :
Veterinary Research Forum; Autumn2020, Vol. 11 Issue 4, p385-391, 7p
Publication Year :
2020

Abstract

Sheep keepers need suitable strategies to improve animal immunity and the quality of their products. This study was aimed to evaluate the effect of nano-selenium (nano-Se) and conjugated linoleic acid (CLA) on an antioxidant statue, trace minerals, and mRNA expression of glutathione peroxidase 1 (GPX1) and selenoprotein W1 (SEPW1) genes in the liver and peroxisome proliferator-activated receptor-gamma (PPARĪ³) and stearoyl COA desaturase 1 (SCD1) genes in fat-tail of male Moghani lambs. Thirty male Moghani lambs, three months old and average weight 30.00 ± 0.25 kg, were assigned to a completely randomized design in a 2×3 factorial arrangement with dietary supplementation of nano-Se (0, 1.00 and 2.00 mg kg-1 dry matter) and CLA (0.00 and 15.00 g kg-1 dry matter). The lambs were slaughtered at the end of the experiment, on day 90 of the experiment. Results showed that dietary inclusion of nano-Se significantly improved antioxidant enzymes glutathione peroxidase and superoxide dismutase in blood, however, did not show any differences in trace mineral treatments. The analysis of qPCR showed that nano-Se inclusion at the highest level (2.00 g kg-1 dry matter) enhanced gene expression of GPX1 (0.64 vs 0.34) and SEPW1 (0.72 vs 0.35) in the liver. Dietary inclusion of CLA increased the expression of PPARĪ³ (0.63 vs 0.38) and decreased SCD1 (0.63 vs 0.33) genes in fat-tail. It could be concluded that selenium inclusion in the growing lamb's diet could improve antioxidant status, however, no synergistic interaction was observed along with CLA on the mentioned parameters. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20088140
Volume :
11
Issue :
4
Database :
Complementary Index
Journal :
Veterinary Research Forum
Publication Type :
Academic Journal
Accession number :
148030707
Full Text :
https://doi.org/10.30466/vrf.2018.93751.2264