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Study the regulatory role of STAT3 on CD8+ T cells using adoptive transfer model in immunodeficient mice.
- Source :
- Journal of Shanghai Jiaotong University (Medical Science); nov2020, Vol. 40 Issue 11, p1437-1446, 10p
- Publication Year :
- 2020
-
Abstract
- Objective * To investigate the intrinsically regulatory role of transcription factor STAT3 on the activation and proliferation of CD8<superscript>+</superscript> T cells in immunodeficient Rag1<superscript>-/-</superscript> mice. Methods * The CD8<superscript>+</superscript>CD44<superscript>-</superscript>CD62L<superscript>+</superscript>Naïve T cells, from CD45.1 wild type (WT) mice and CD45.2-CD8 (ΔStat3) mice, were sorted by flow cytometry cell sorter, equally mixed and labelled with Carboxyl Fluorescein diacetate Succinimidyl Ester (CFSE), and then transferred (i.v.) to the Rag1<superscript>-/-</superscript> mice. Ten days after CD8<superscript>+</superscript> T cells transfer, the proportion and proliferation of CD8<superscript>+</superscript> T cells in the spleen, mesenteric lymph nodes (mLNs) and popliteal lymph nodes (pLNs) were determined by flow cytometry. TNF-α, IFN-γ, FasL and granzyme B (GraB) produced by the CD8<superscript>+</superscript> T cells were measured with flow cytometry after PMA and ionomycin stimulation. The statistical significance of activation and effectors expressed between WT and ΔStat3 CD8<superscript>+</superscript> T cell was analyzed by t test. Results * The percentages of WT CD8<superscript>+</superscript> T cells in spleen, mLNs and pLNs were significantly higher than that in ΔStat3 CD8<superscript>+</superscript> T cells (76.2% vs 23.4%, 82.1% vs 17.4%, 64.5% vs 32.3%, with all P=0.008). The percentage and cell number of activated CD44<superscript>+</superscript>CD8<superscript>+</superscript> T cells in WT CD8<superscript>+</superscript> T cells were much higher than those in ΔStat3 CD8<superscript>+</superscript> T cells in pLNs (all P=0.008). In spleen, pLNs and mLNs, the levels of TNF-α, IFN-γ, GraB and CD107a expressed in WT CD8<superscript>+</superscript> T cells were higher than those in ΔStat3 CD8<superscript>+</superscript> T cells (all P<0.05). Conclusion · Rag1<superscript>-/-</superscript> mice can work as an ideal model to evaluate the survival, proliferation, activation and function of CD8<superscript>+</superscript> T cells in vivo. STAT3 intrinsically regulates the proliferation, activation and function of CD8<superscript>+</superscript> T cells in vivo. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 16748115
- Volume :
- 40
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Journal of Shanghai Jiaotong University (Medical Science)
- Publication Type :
- Academic Journal
- Accession number :
- 148023706