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TOP2A/MCM2, p16INK4a, and cyclin E1 expression in liquid-based cytology: a biomarkers panel for progression risk of cervical premalignant lesions.

Authors :
Del Moral-Hernández, Oscar
Hernández-Sotelo, Daniel
Alarcón-Romero, Luz del Carmen
Mendoza-Catalán, Miguel Angel
Flores-Alfaro, Eugenia
Castro-Coronel, Yaneth
Ortiz-Ortiz, Julio
Leyva-Vázquez, Marco Antonio
Ortuño-Pineda, Carlos
Castro-Mora, Wendy
Illades-Aguiar, Berenice
Source :
BMC Cancer; 1/7/2021, Vol. 21 Issue 1, p1-13, 13p
Publication Year :
2021

Abstract

<bold>Background: </bold>To improve the efficiency of early diagnosis systems for cervical cancer, the use of cellular and viral markers for identifying precancerous lesions with a greater probability to progress to cancer has been proposed. Several cellular proteins and markers of oxidative DNA damage have been suggested as possible biomarkers of cervical carcinogenesis; however, they have not been evaluated together. In this study, we analyzed the expression of the cellular markers p16INK4a, Ki-67, CyclinE1, TOP2A/MCM2, and telomerase, as well as the DNA oxidative damage markers ROS and 8-OHdG. The analyses were performed in liquid-based cervical cytology samples or biopsies with premalignant lesions or cervical cancer diagnosis, with the purpose of selecting a panel of biomarkers that allow the identification of precursor lesions with greater risk of progression to cervical cancer.<bold>Methods: </bold>We analyzed 1485 liquid-based cytology samples, including 239 non-squamous intraepithelial lesions (NSIL), 901 low-grade squamous intraepithelial lesions (LSIL), 54 high-grade squamous intraepithelial lesions (HSIL), and 291 cervical cancers (CC). The biomarkers were analyzed by immunocytochemistry and Human Papilloma Virus (HPV) genotyping with the INNO-LiPA genotyping Extra kit.<bold>Results: </bold>We found that all tested cellular biomarkers were overexpressed in samples with high risk-HPV infection, and the expression levels increased with the severity of the lesion. TOP2A/MCM2 was the best biomarker for discriminating between LSIL and HSIL, followed by p16INK4a and cyclinE1. Statistical analysis showed that TOP2A/MCM2 provided the largest explanation of HSIL and CC cases (93.8%), followed by p16INK4a (91%), cyclin E1 (91%), Ki-67 (89.3%), and telomerase (88.9%).<bold>Conclusions: </bold>We propose that the detection of TOP2A/MCM2, p16INK4a and cyclin E1 expression levels is useful as a panel of biomarkers that allow identification of cervical lesions with a higher risk for progression to CC with high sensitivity and precision; this can be done inexpensively, in a single and non-invasive liquid-based cytology sample. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712407
Volume :
21
Issue :
1
Database :
Complementary Index
Journal :
BMC Cancer
Publication Type :
Academic Journal
Accession number :
147996042
Full Text :
https://doi.org/10.1186/s12885-020-07740-1