THE ROLE OF SHISHA SMOKE IN CAUSING HISTOPATHOLOGICAL AND TERATOGENIC EFFECTS ON PREGNANT MICE AND THEIR EMBRYOS.

The purpose of present study was to evaluate the toxic, teratogenic and histopathological effects of shisha smoking on some organs (uterine horns, liver, lung, heart and kidneys) of pregnant white mice Mus musculus and their fetus. Twenty pregnant female mice were used and they were divided into twice experimental groups. The first one as a control group was exposed to fresh air, while the second was exposed to shisha smoke once daily at 7th to 18th gestation day for 30 minutes for each exposed period per day. Exposed mice to shisha smokes showed increase locomotors activity. Fetal malformations were recorded at rate 86% appearance of malformed fetuses, like water nymph, aborted embryos, head defects with cephalomegaly, meningomeningo myelocele, sunken eye, cyanotic skin, skeletal deformity. Histopathological lesions in pregnant mice organs included, infiltration of inflammatory mononuclear cells, accumulation of serous fluid between the pulmonary alveoli, coagulated necrosis and thickening into alveolar septa, necrosis in the myocardial fibers, pyknosis, binuclated hepatocytes megakaryocyte. The histopathological effects in fetal organs were similar to that of the pregnant mice. In conclusion, shisha smokes increase locomotor activity, fetal malformations with prominent histopathological features in multiple organs pregnant mice and their fetuses. [ABSTRACT FROM AUTHOR]