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A natural oil increases specific anti‐OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide.

A natural oil increases specific anti‐OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide.

Authors :
Campra, Noelia Anahí
Montironi, Ivana Dalila
Reinoso, Elina Beatriz
Raviolo, José
Moreno, Federico Ruiz
Maletto, Belkys
Cariddi, Laura Noelia
Source :
Journal of Leukocyte Biology; Jan2021, Vol. 109 Issue 1, p223-232, 10p
Publication Year :
2021

Abstract

The efficacy of certain vaccines is improved by the use of adjuvants. Nowadays, the development of new, effective, and safe adjuvants that stimulate the innate immune response is researched. In this context, medicinal plants appear as a suitable alternative. Minthostachys verticillata essential oil (EO) has demonstrated the ability to modulate mechanisms of the innate immune response. Thus, the present work aimed to evaluate the EO adjuvant effect on humoral and cellular immunity, coadministered with OVA as antigen. The chemical analysis of EO by gas chromatography–mass spectrometry revealed a predominant pulegone–menthone chemotype. EO (1.25, 2.5, or 5.0 mg/ml) did not alter the viability of murine fibroblasts (3T3 cell line) neither showed signs of toxicity in Balb/c mice inoculated subcutaneously. The serum of mice immunized with OVA + EO showed increased levels of anti‐OVA‐specific antibodies of IgG1 subclass compared with the mice immunized with OVA alone revealing an adjuvant effect of EO. The delayed type hypersensitivity showed that the combination OVA + Al(OH)3 + EO was the best to induce a cellular immune response that extended until 48 h postinjection of OVA. M. verticillata EO appears as a new, safe, and effective adjuvant, which should continue to be studied for their possible future incorporation into vaccine formulations. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07415400
Volume :
109
Issue :
1
Database :
Complementary Index
Journal :
Journal of Leukocyte Biology
Publication Type :
Academic Journal
Accession number :
147772933
Full Text :
https://doi.org/10.1002/JLB.3AB0720-093RR