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miR-155 influences cell-mediated immunity in Balb/c mice treated with aflatoxin M1.
- Source :
- Drug & Chemical Toxicology; Jan2021, Vol. 44 Issue 1, p39-46, 8p
- Publication Year :
- 2021
-
Abstract
- Aflatoxin M<subscript>1</subscript> (AFM<subscript>1</subscript>) is a 4-hydroxylated metabolite of aflatoxin B<subscript>1</subscript> (AFB<subscript>1</subscript>). It induces various toxicological effects including immunotoxicity. In the present study, we investigated the effects of AFM<subscript>1</subscript> on immune system and its modulation by MicroRNA (miR)-155. AFM<subscript>1</subscript> was administered intraperitoneally at doses of 25 and 50 µg/kg for 28 days to Balb/c mice and different immune system parameters were analyzed. The levels of miR-155 and targeted proteins were evaluated in isolated T cells from spleens of mice. Spleen weight was reduced in mice exposed to AFM<subscript>1</subscript> compared to negative control. Proliferation of splenocytes in response to phytohemagglutinin-A was reduced in mice exposed to AFM<subscript>1</subscript>. IFN-γ was decreased in mice exposed to AFM<subscript>1</subscript>, whereas IL-10 was increased. Concentration of IL-4 did not change different in mice exposed to AFM<subscript>1</subscript> compared to negative control. Exposure to AFM<subscript>1</subscript> reduced the expression of miR-155. Significant upregulation of phosphatidylinositol-3, 4, 5-trisphosphate 5-phosphatase 1 (Ship1) and suppressor of cytokine signaling 1 (Socs1) was observed in isolated T cells from spleens of mice treated with AFM<subscript>1,</subscript> but the transcription factor Maf (c-MAF) was not affected. These results suggest that miR-155 and targeted proteins might be involved in the immunotoxicity observed in mice exposed to AFM<subscript>1</subscript>. The Immunotoxic effects of AFM<subscript>1</subscript> on T cell functions. Exposure to AFM<subscript>1</subscript> reduced the expression of miR-155. Significant upregulation of Ship1 and Socs1 was observed in isolated T cells from spleens of mice treated with AFM<subscript>1,</subscript> but the c-MAF was not affected. Since Ship1 is a functional target of miR-155 that modulates production of IFN-γ, miR-155 may play a role in AFM<subscript>1</subscript>-induced Th<subscript>1</subscript> response suppression (DTH) through targeting of this protein (↑ increase; ↓ decrease). AFM<subscript>1</subscript> inhibited cell-mediated immunity. Exposure of mice to AFM<subscript>1</subscript> resulted in a significant decrease in expression of miR-155. Exposure of mice to AFM<subscript>1</subscript> resulted in up-regulation of Ship1 and Socs1. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01480545
- Volume :
- 44
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Drug & Chemical Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 147626532
- Full Text :
- https://doi.org/10.1080/01480545.2018.1556682