Back to Search
Start Over
High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai.
- Source :
- International Journal of Molecular Sciences; Nov2020, Vol. 21 Issue 21, p7896, 1p
- Publication Year :
- 2020
-
Abstract
- Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C<subscript>15</subscript>H<subscript>16</subscript>N<subscript>3</subscript>O<subscript>2</subscript>S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine. [ABSTRACT FROM AUTHOR]
- Subjects :
- METASTASIS
CELL motility
BIOLUMINESCENCE
GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 21
- Issue :
- 21
- Database :
- Complementary Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 147311346
- Full Text :
- https://doi.org/10.3390/ijms21217896