Study on Chemical Constituents of Ethanol Extract from Yao Medicine Cissampelopsis spelaeicola.

[Objectives] The research aimed to study the chemical constituents of ethanol extract from Yao medicine Cissampelopsis spelaeicola. [Methods] The petroleum ether, ethyl acetate and n-butanol fractions from 75% ethanol extract of C. spelaeicola were isolated and purified by silica gel, SephadexLH-20 gel column and AB-8 macroporous resin column, etc. The structures of the compounds were analyzed and identified by physicochemical properties and spectral data（mass spectrometry, hydrogen spectrum and carbon spectrum）. [Results] Twelve compounds were isolated and identified from 75% ethanol extract of C. spelaeicola. β-sitostero（I） and stigmasterol（II） were isolated from petroleum ether fraction; p-hydroxybenzoic acid（III）, β-daucossterol（IV）, protocatechuic acid（V）, 6β-hydroxyeremophi-7（11）-en-12, 8β-olide（VI）, 10β-hydroxyeremophil-7（11）-en-8, 12-olide（VII）, 10β-hydroxyeremophi-7（11）, 8（9）-dien-8, 12-olide（VIII）, quercetin（IX）, hyperin（X） and 4α-hydroxy-eudesman-11-ene（XI） were isolated from ethyl acetate fraction; quercetin-3-O-robinobioside（XII） was isolated from n-butanol fraction. Compounds I-XII were isolated from C. spelaeicola for the first time. [Conclusions] The study can lay material foundation for activity study of C. spelaeicola. [ABSTRACT FROM AUTHOR]