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Regulation effects of total flavonoids in Morus alba L. on hepatic cholesterol disorders in orotic acid induced NAFLD rats.
- Source :
- BMC Complementary Medicine & Therapies; 8/17/2020, Vol. 20 Issue 1, p1-12, 12p, 3 Charts, 4 Graphs
- Publication Year :
- 2020
-
Abstract
- Background: Mulberry leaves are the dried leaves of Morus alba L., flavonoids from mulberry leaves (MLF) has showed regulatory effect on abnormal lipid metabolism, but the regulatory mechanism of MLF on cholesterol metabolism is still missing. This study was designed to investigate the effect of MLF and its active metabolite quercetin on regulating cholesterol disorders. Methods: The mechanism of MLF on alleviating liver injury and regulating cholesterol was examined in dyslipidemic SD rats. The regulatory mechanism of quercetin for cholesterol disorders have also been detected through lipid laden HepG2 cell model. Results: Our results showed that MLF significantly inhibited lipid accumulation and alleviate hepatic injury in NAFLD rat model. The hepatic expression level of SREBP2, HMGCR and miR-33a were significantly down-regulated, while CYP7A1 was induced by MLF treatment. In vitro, Quercetin significantly decreased lipid accumulation in HepG2 cells. Mechanistically, quercetin could inhibit the mRNA and protein expression level of SREBP2 and HMGCR with or without LDL treatment. In addition, quercetin could also reduce the LXRβ while induced SR-BI mRNA expression. Conclusion: Our findings indicate that MLF and quercetin could reduce the excessive cholesterol accumulation in vivo and in vitro. These cholesterol-regulating phenomenon might attribute to its effect on down-regulating the expression of lipid-related markers such as SREBP2 and HMGCR, which may exert a protective role in the NAFLD treatment. [ABSTRACT FROM AUTHOR]
- Subjects :
- FATTY liver prevention
CHOLESTEROL metabolism
ACIDS
ANIMAL experimentation
BIOLOGICAL assay
BIOLOGICAL models
CARRIER proteins
CELL culture
CELL lines
CHOLESTEROL
FLAVONOIDS
GENE expression
HETEROCYCLIC compounds
HISTOLOGY
IMMUNOHISTOCHEMISTRY
LEAVES
LIPIDS
LIVER
MESSENGER RNA
METABOLISM
POLYMERASE chain reaction
RATS
STAINS & staining (Microscopy)
WESTERN immunoblotting
PLANT extracts
DATA analysis software
CELL survival
IN vitro studies
ONE-way analysis of variance
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 20
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 147104130
- Full Text :
- https://doi.org/10.1186/s12906-020-03052-w