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Osteogenic Potential of Fast Set Bioceramic Cements: Molecular and In Vitro Study.

Authors :
Pasqualini, Damiano
Comba, Allegra
Annaratone, Laura
Mola, Virginia
Alovisi, Mario
Breschi, Lorenzo
Mazzoni, Annalisa
Scotti, Nicola
Cassoni, Paola
Berutti, Elio
Source :
Applied Sciences (2076-3417); Oct2020, Vol. 10 Issue 19, p6713, 11p
Publication Year :
2020

Abstract

Featured Application: Bioceramic materials have been recently proposed for several applications in endodontic practice. The impact of their setting time and chemical composition on biocompatibility is still controversial. This in vitro study evaluated the osteogenic potential of different bioceramic formulations. Recently, pre-mixed bioceramics in fast set formulations have been increasingly utilized in clinical practice as an alternative to mineral trioxide aggregate (MTA) for their shorter setting time and better handling properties. However, the impact on their osteogenic potential, due to modifications in chemical composition to promote a fast setting, is still unclear. This molecular and in vitro study compared the osteogenic potential of root repairing material putty fast set (FSP) with root-repairing material putty (RRMPU), root-repairing material paste (RRMPA), Biodentineā„¢ and MTA. The null hypothesis tested was that there are no differences among the tricalcium silicate materials in terms of osteogenic potential. Standardized discs were cultured with MG-63 human osteoblastic-like cells to assess biocompatibility, the activity of alkaline phosphatase (ALP) and osteogenic potential. Biocompatibility was evaluated at baseline and after 24 and 48 h. Osteogenic differentiation was assessed after 15 days. Data were analyzed with one-way ANOVAs and Tukey's post-hoc test (p < 0.05). All materials showed biocompatibility and bioactivity. ALP activity, which induces mineral nodule deposition, increased in all the cements tested, with a significant increase in RRMPU (p < 0.001) and FSP (p < 0.001) samples versus MTA. In vitro mineralization was significantly increased for RRMPU (p < 0.0001), FSP (p = 0.00012) and Biodentineā„¢ (p < 0.0001) versus MTA. The bioceramics tested showed higher levels of biocompatibility and bioactivity than MTA; a higher capacity for mineralization was observed with RRMPU and FSP versus MTA. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20763417
Volume :
10
Issue :
19
Database :
Complementary Index
Journal :
Applied Sciences (2076-3417)
Publication Type :
Academic Journal
Accession number :
147002692
Full Text :
https://doi.org/10.3390/app10196713