STUDY OF DIFFERENT FACTORS EFFECTED IN PRODUCTION OF DEXTRANASE ENZYME FROM A LOCAL ISOLATE OF B. SUBTILIS Z2 BACTERIA.

The present study aims to detection optimal conditions of production of dextranase enzyme from isolate of B. subtilis Z2. Eight carbonic sources were represented by Dextran, Molasses, fructose, sucrose, glucose, Lactose, CMC and Arabinose) at concentration of 0.5% for each source. It was found that the best was represented by Molasses carbonic which showed higher activity and qualitative activity of 7.11 units / ml and 83.01 units / mg. The nitrogen sources were selected, including yeast extract, peptone, yeast extract with peptone, gelatin, meat extract, Ammonium sulphate, Potassium nitrate and Ammonium chloride. These sources were added at a concentration of 0.5% to the production medium. The Peptone with yeast extract source was the best of used nitrogenic sources at concentration of 1.2%, which showed higher activity and qualitative activity at 8.89 units / ml and 85.5 units / mg. The best hydrogen number was 7. The size of inoculums of 3 x 107 c.f.u. was the best which showed higher activity and qualitative activity of 21.702 units/ ml and 301.29 units/ mg. there incubation which 44 hours showed higher activity and qualitative activity of 32.934 units / ml and 633.52 units / mg. [ABSTRACT FROM AUTHOR]