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Alginate oligosaccharide protects endothelial cells against oxidative stress injury via integrin-α/FAK/PI3K signaling.
- Source :
- Biotechnology Letters; Dec2020, Vol. 42 Issue 12, p2749-2758, 10p
- Publication Year :
- 2020
-
Abstract
- Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H<subscript>2</subscript>O<subscript>2</subscript>-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mechanisms. HUVECs were treated with 100–800 µM hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>) for various periods (12, 24, and 36 h) to establish an in vitro oxidative stress and apoptosis HUVEC model. AOS protects HUVEC cells against oxidative stress-induced apoptosis by decreasing the expression levels of caspase 3 and Bax, and increasing Bcl-2 expression. Microarray assay, real-time PCR and western blot results revealed that AOS was able to effectively suppress H<subscript>2</subscript>O<subscript>2</subscript>-induced apoptosis via regulated integrin-α/FAK/PI3K pathway by influencing the expression of integrin-α, FAK, PI3K, PTEN, P21, and CDK2. In conclusion, our study suggests that AOS can protect endothelial cells against oxidative stress injury caused by H<subscript>2</subscript>O<subscript>2</subscript>, providing novel alternative strategies to prevent atherosclerosis in the future. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENDOTHELIAL cells
OXIDATIVE stress
ALGINIC acid
APOPTOSIS
UMBILICAL veins
Subjects
Details
- Language :
- English
- ISSN :
- 01415492
- Volume :
- 42
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Biotechnology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 146605399
- Full Text :
- https://doi.org/10.1007/s10529-020-03010-z