Development of a dual-gene PCR assay for simultaneous amplification of structural and non-structural genes of bovine rotavirus A.

Rotavirus can be detected in stool samples by using serological and molecular methods. An easy and rapid dual gene RTPCR assay for the simultaneous amplification of the capsid protein VP4 (structural) and rotavirus enter toxin NSP4 (nonstructural protein) has been developed. We validated the assay by comparing the results of single target PCR with dual-gene PCR products. [ABSTRACT FROM AUTHOR]