Genetic profiling of breast cancer: A case study on Wnt and BRCA pathways.

Breast cancer prevalence tends to increase by the time estimated at ± 1,050,346 cases yearly. There are reports of genetic factors as the main reasons, yet there is poor information on this aspect in Indonesia. Wnt and BRCA pathways had been understood to regulate the development of breast cancer. The mutation of BRCA family members had been found in many breast cancers cases. Meanwhile, Wnt family demostrate its two faces. Some canonical Wnt members are playing on promoting tumorigenesis, while some non-canonical Wnts antagonizing the canonical one. This study aimed to unveil the genetic profile of breast cancer tissue isolated from East Javan patients in Indonesia with focus on Wnt and BRCA pathways. Furthermore, we seek the possible role of mainly Wnts and BRCA family members genes in breast cancer cases found in East Java. Total RNA of stages III and IV fresh biopsy samples from five patients were isolated and reverse-transcripted into cDNA. The expression Wnt4, Wnt5a, BRCA2, and TP53 were analyzed using relative q- PCR with standardized OneWay ANOVA. This study revealed that TP53, BRCA2, were expressed highly in earlier stage (stage IIIB) along with Wnt4, while Wnt5a was highly expressed in later stage (stage IV). From this study we conclude that Wnt4 was supposed to be responsible for earlier cancer development, while Wnt5a was supposed to be responsible for later development. We also suggested that both TP53 and BRCA2 might bear some mutations Currently, we are working on this matter. [ABSTRACT FROM AUTHOR]