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Rapid screening of marine bacterial symbionts using MALDI-TOF MS.
- Source :
- Archives of Microbiology; Oct2020, Vol. 202 Issue 8, p2329-2336, 8p
- Publication Year :
- 2020
-
Abstract
- Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria. MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups' identification. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03028933
- Volume :
- 202
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Archives of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 145372794
- Full Text :
- https://doi.org/10.1007/s00203-020-01917-9