Phytochemical Incorporated Spunned Nanofibrous Polycaprolactone Scaffold for Stem Cell Loading and Drug Delivery Applications.

The merging of traditional medicine with modern technology in the building up of novel scaffold devices, is of additional relevance in this era of regenerative medicine. Hence, the aim of this study is to fabricate and test the safety and efficacy of the flavonoid Naringenin, incorporated Polycaprolactone (Nar-PCL) electrospun nano fibrous scaffolds intended for drug delivery and as stem cell loading stations. Electro spun mats of 5% and 20% wt/wt Naringenin-PCL was fabricated and physico-chemically characterisated by FTIR, NMR spectroscopy, XRD analysis and SEM. In vitro drug release of Naringenin from spun mats were studied in PBS for 3, 6, 12 days spectrophotometrically at 292 nm. L929 fibroblasts were grown over Nar-PCL and control PCL scaffolds and Cell adhesion was demonstrated by SEM,viability by Calcien AM- Ethidium homodimer staining, and proliferation by MTT and LDH assay. Simultaneously, viability and adhesion of isolated rat Adipose derived Mesenchymal stem cells (ADMSCs) on different scaffolds were also assessed. Antibacterial activity of Naringenin against Psuedomonas Aeruginosa and Staphylococcus aureus was analysed by Kirby-Bauer method. Finally, the combination product of 5% wt/wt Nar- PCL portrayed better cytocompatible, regenerative and antibacterial properties of Naringenin, proving as a prospective candidate for stem cell loading and drug delivery. [ABSTRACT FROM AUTHOR]