蛍光検出器付HPLC を用いた鶏卵および卵加工食品中の5 種類の アフラトキシン一斉分析法の検討

We examined the optimum extraction solvent and conditions for fluorescence derivatization for a simultaneous analytical method that uses HPLC-FLD to measure five aflatoxins (AFB1, AFB2, AFG1, AFG2, and AFM1) in egg and processed egg samples. An egg sample and a processed egg sample were extracted under weakly acidic conditions using 0.1% formic acid in acetonitrile / water (9:1), cleaned up with an immunoaffinity column, and derivatized to fluoresce using trifluoroacetic acid (TFA) and n-hexane under warming conditions. The method was validated by replicate analysis of egg samples and granulated whole egg powder samples spiked with each AFB1, AFB2, AFG1, AFG2, and AFM1 at 0.1, 0.5 and 5.0 μg/kg. The recoveries of the egg samples and the granulated whole egg powder samples were 85.1-93.2% and 82.7-88.9%, respectively. The relative standard deviation (RSD) of repeatability and intermediate precision were ≤ 5.7% and ≤ 7.8%, respectively, all samples. The method detection limit (MDL) and the method quantification limit (MQL) were 0.004-0.010 μg/kg and 0.014-0.035 μg/kg, respectively, and MQL of the proposed method was lower than LOQ of official method in Japan. The proposed method was applied to four commercial egg samples and 19 processed egg samples, and no AFs were detected. [ABSTRACT FROM AUTHOR]